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(PDF) Degradation of Castor Oil and Lipase Production by

Degradation of Castor Oil and Lipase Production by Pseudomonas aeruginosa. Article (PDF Available) January 2009 with 513 Reads How we measure 'reads' A 'read' is counted each time someone views

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Degradation of castor oil and lipase production by

Two strains of Pseudomonas aeruginosa were used to produce lipase from castor oil aiming to control the different castor oil wastes and technical lipase production. Both strains are able to grow on media consisting of 20 g/litre of Castor oil and 3 g/litre of yeast extract at 37°C. The lipase enzyme activity at 37, 60 and 75°C in the presence of 0.4, 0.8, 2.0 and 2.8 mg/ml of p-nitrophenyl...

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Cited by: 12

Degradation of Castor Oil and Lipase Production by

Degradation of Castor Oil and Lipase Production by Pseudomonas aeruginosa 1 Amro A. Amara and 2Soheir R. Salem Protein Research Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt 2 Biological and Geological Science Department, Faculty of Education, Alexandria University,

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Degradation of Castor Oil and Lipase Production by

In this study, two strains of P. aeruginosa were used to produce lipase from Castor oil aiming to control the different castor oil wastes and technical lipase production. Both strains are able to grow on media consists of 20 g/l Castor oil and 3 g/l Yeast extract at 37°C. The lipase enzyme activity at 37, 60 and 75°C in presence of 0.4, 0.8

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Degradation of Castor Oil and Lipase Production by

Degradation of Castor Oil and Lipase Production by Pseudomonas aeruginosa . By Amro A. Amara and Soheir R. Salem. Abstract. Abstract: Many Enzymes produced by Pseudomonas aeruginosa have been used in commercial scales, lipase is an example. P. aeruginosa has been investigated intensively during past decays regarding its medical and industrial importance. It will establish in different wastes

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Amro A. Amara and Soheir R. Salem

Selection of potential lipase producer for castor oil

Castor oil, also known as ricinus oil, occurs in the seeds of the castor plant, Ricinus communis. Fifteen isolates were screened for lipase production on solid and liquid media. The isolate which

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Selection of potential lipase producer for castor oil

Castor oil, also known as ricinus oil, occurs in the seeds of the castor plant, Ricinus communis. Fifteen isolates were screened for lipase production on solid and liquid media. The isolate which

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Biosynthesis of γ-Decalactone with Lipase-Catalyzed

Castor oil is the main feedstock to produce γ-decalactone among many materials. In this paper, an approach to efficiently improve the production of γ-decalactone by adding porcine pancreatic lipase was described. Saccharomyces cerevisiae strain with the highest production efficiency of γ-decalactone was firstly selected from ten yeasts. Then, the culture conditions were optimized.

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Impact of Lipase-Mediated Hydrolysis of Castor Oil on c

acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concen-trations on the c-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L-1 was found to be the optimal oil concentration. Under these conditions, cells were able to produce lipase

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Production and Characterization of Biodiesel Using

The effects of reaction time (24–120 h), temperature (40 and 60°C), oil to methanol molar ratio (1 : 1 to 1 : 6), and immobilized lipase amount (1–7% of oil weight) on biodiesel production were investigated in the reaction system. The reaction mixture was washed with distilled water to remove the glycerol and excess methanol.

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Bioreactor operated production of lipase: Castor oil

Vol. 37, May 1999, pp. 481-486 Bioreactor operated production of lipase: Castor oil hydrolysis using partially-purified lipase Chetna Sharon, Midori Nakazato, Hiroaki I. Ogawa & Yasuhiko Kato Dept. of Applied Chemistry, Kyushu Institute of Technology, I-I Sensui-cho, Tobata-ku, Kitakyushu-shi

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发布位置:Indian Journal of Experimental Biology 1999Chetna Sharon Midori Nakazato Hiroaki I Ogawa Yasuhiko Kato

Lipase Activity in Fermented Oil Seeds of Africa Locust

Liman et al., Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).137 those that yield gum are of economic importance (Dupriez, 1984). Ricinus communis (Castor oil seed) is of the spurge family widely cultivated as an

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Monitoring the Rhizopus oryzae lipase catalyzed

The enzymatic hydrolysis of castor oil using lipase R. oryzae was successfully carried out at mild reaction conditions and the maximum transformation of castor oil (FFA 90%) was achieved at only 0.1% (w/v) of enzyme concentration at 12 h of incubation period. The developed PLS model showed better performance in terms of correlation coefficient

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New Colorimetric Method for Lipases Activity Assay

tained from the relation between lipase concentration and the corresponding absorbance values determined at 750 nm was linear for the concentration ranges of 0 to 0.8 g L−1. [5] A. A. Amara and S. R. Salem, “Degradation of Castor Oil and Lipase Production by Pseudomonas aeruginosa,” American-Eurasian Journal of Agricultural & Environ-

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Lipase-Secreting Bacillus Species in an Oil-Contaminated

Thus, in this study, samples from night market were chosen for screening lipase producing bacteria in the oil-contaminated soil, which can be potential microbial-based degradation approach and an “extraction pool” for the extracellular enzymes. Moreover, the differential secretion of the lipase and protease enzyme was also analyzed. Lipase and protease are the class of enzymes that perform

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Study of Soybean Oil Hydrolysis Catalyzed by

Lima studied biodiesel production from soybean and castor bean oils, using the process of hydroesterification catalyzed by niobic acid in pellets, and obtained conversion rates of 84% for the reaction of hydrolysis of soybean oil and 82% for castor bean oil; for the esterification reactions, the highest conversion rates were 92% for FFAs from

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Monitoring the Rhizopus oryzae lipase catalyzed

The enzymatic hydrolysis of castor oil using lipase R. oryzae was successfully carried out at mild reaction conditions and the maximum transformation of castor oil (FFA 90%) was achieved at only 0.1% (w/v) of enzyme concentration at 12 h of incubation period. The developed PLS model showed better performance in terms of correlation coefficient

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Lipase-Secreting Bacillus Species in an Oil-Contaminated

Thus, in this study, samples from night market were chosen for screening lipase producing bacteria in the oil-contaminated soil, which can be potential microbial-based degradation approach and an “extraction pool” for the extracellular enzymes. Moreover, the differential secretion of the lipase and protease enzyme was also analyzed. Lipase and protease are the class of enzymes that perform

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Isolation and characterization of lipase producing

parameters for maximum production of lipase for degradation of oil stains. Results pertaining to the isolations, lipolytic potentials and production of lipase by selected strains are presented in this paper. Materials and Methods Samples collection Isolation of lipolytic bacteria was made from three types of soil samples: i. Normal soils, ii. Oil contaminated soils and iii. Stored oil samples

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Effect of POX genotype and Lip2p overexpression on lactone

Castor reconsumption oil-Decalactone strain. production Lipase Yarrowia lipolytica a b s t r a c t-Decalactone production from ricinoleic acid biotransformation by Yarrowia lipolytica has drawn the attention of many authors and, in the last years, molecular and physiological developments were made

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发布位置:Process Biochemistry 2015Adelaide Braga A M Crutzle Coq A M Crutzle Coq Remi Dulermo Remi Dulermo从属关系: University of Minho Agro Paristech Institut National De La Recherche Agronomique关于:Lipase Castor oil Beta oxidation

Extracellular lipase production by Penicillium citrinum

amended to the lipase production medium at 1% each. Olive oil, Sunflower oil, Castor oil, Mustard oil and Groundnut oil were used as Results and discussion The influence of the environmental factors such as temperature, pH, nitrogen, carbon and lipid sources on lipase production has been studied by various investigators. These factors are

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Screening of Microorganisms Isolated from Different

Screening of Microorganisms Isolated from Different Environmental Samples for Extracellular Lipase Production Haliru Musa and Bukola Christianah Adebayo-Tayo Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria E-mail: <[email protected]; [email protected] Abstract

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发布位置:AU Journal of Technology 2012Haliru Musa B C Adebayotayo从属关系: University of Ibadan关于:Bacteria Lipase

Production and Characterization of Biodiesel Using

A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of

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Ronald M Atlas,1993

extracellular lipase from Mucor sp. strain isolated from palm fruit. Enzyme Microb. Technol., 31: 968-975. Adenipekun, C. O and L. Q. Kassim, 2006. Effects of spent engine oil on the growth parameters and moisture content of Celosia argentea. Proceeding of the 15th Annual Botanical Society of Nigeria Conference, University of Uyo, 108-111.

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Produccion De Lipasa Por Pseudonomas Ensayos 3773

In this study, two strains of P. aeruginosa were used to produce lipase from Castor oil aiming to control the different castor oil wastes and technical lipase production. Both strains are able to grow on media consists of 20 g/l Castor oil and 3 g/l Yeast extract at 37°C. The lipase enzyme activity at 37, 60 and 75°C in presence of 0.4,

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New Colorimetric Method for Lipases Activity Assay

During the lipase production, it is important to determine the lipase activity by a simple and rapid method to select the best condition for the production. Although several methods have been developed for the determination of lipase activity, only two methods are commonly used in the literatures.

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New Colorimetric Method for Lipases Activity Assay

A simple, rapid and precise method has been developed for determination of lipase activity in microbial media. The method is based on using phenyl acetate as substrate for lipase and determination of liberated phenol by Folin Ciocalteu reagent. Reaction mixture containing substrate 2.4 ml of phenyl acetate 165 μM in Tris HCl buffer, 0.1 M and pH 7, with 1% (v/v) Triton X-100) and 0.1 ml

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The Production of γ-Decalactone by Fermentation of

2009-07-11 A microbial process for the production of optically-active γ-decalactone from the ricinoleic acid present as triglycerides in castor oil has been developed, γ-decalactone (γDL) is a component of some fruit flavours, being an important organoleptic component of peach flavours.

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Fermentative Production of Microbial Enzymes and their

1. Amro A, Soheir SR. Degradation of castor oil and lipase production by Pseudomonas aeruginosa. Journal of Agricultural & Environmental Sciences. 2009; 5(4):556–563. 2. Ahlawat S,Dhiman SS, Battan B,Mandhan RP,Sharma J. Pectinase production by Bacillus subtilis and its potential application in biopreparation of cotton and micropoly fabric

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Isolation, Identification and Characterization of a Lipase

The other purposes of this study are production, partial purification, characterization of lipase activity at different pH and incubation time, production and determination of molecular weight analysis. The lipase was partially purified up to 30% saturation using ammonium sulphate precipitation. Purity of lipases was cheeked by SDS-PAGE

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Changes in enzyme activities during the fermentation of

production and consumption of ogiri from castor oil seed is decreasing and being replaced by imported bouillon cubes like maggi cubes (Essien, 1983). Ibe and Orabuike (2009) reported that the microorganisms involved in the fermentation of castor oil bean for ‘ogiri’ production

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Rheological Behavior of Castor Oil Biodiesel Energy & Fuels

This work aims at assessing the rheological behavior of castor oil, castor oil biodiesel, and undegraded and degraded biodiesel at different exposure times and temperatures. Castor oil biodiesel presents viscosity higher than diesel oil, but this drawback can be corrected by means of blends of both components at different proportions. The

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Gas Chromatographic Determination of Glycerol

Gas Chromatographic Determination of Glycerol and Triglycerides in Biodiesel from Jatropha and Castor Vegetable Oils p.436. Electricity Production from Biomass in Nigeria: Options, Prospects and Challenges p.444. Biodiesel Production Using Mixed Solid Catalysts p.451. Evaluation of Solar Water Heating Systems in Botswana p.459. Potential of Biogas Production from Floating Aquatic Weeds

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Biodegradation of polyurethane derived from castor oil

The results suggest that the degradation of polyurethane derived from castor oil occurs. TG curves are used in order to indicate the biodegradation, showing changes between the thermal behavior of the samples that were inoculated with microorganisms and the control samples.

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发布位置:Polimeros-ciencia E Tecnologia 2008Jose M Cangemi Antonia Marli Dos Santos Salvador Claro Neto Gilberto Orivaldo C从属关系: Sao Paulo State University关于:Castor oil Biodegradation Microorganism Polyurethane Polyester

Modification of Process Parameters for Enhanced Lipase

production of cosmetics, and pharmaceuticals. Therefore, a potential lipase producing bacterial strain was isolated and identified as gram +ve Bacillus SR1. Among different oils tested, olive oil was found to be the favorable substrate for lipase induction. Additionally,

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Research review paper Production, purification

manufacture, and production of cosmetics, and pharmaceuticals (Rubin and Dennis, 1997a,b; Kazlauskas and Bornscheuer, 1998). Lipase can be used to accelerate the degradation of fatty waste (Masse et al., 2001) and polyurethane (Takamoto et al., 2001). Major applications of lipases are summarized in Table 2. Most of the industrial microbial

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